Insecticidal Effect of Cry Toxins Produced by Bacillus thuringiensis on Diceraeus melacanthus (Dallas, 1851) and Euschistus heros (Fabricius, 1798) (Hemiptera: Pentatomidae)

Some species of the Heteroptera (Hemiptera) suborder are great agricultural importance, mainly as pests, and their control is often necessary. The use Bacillus thuringiensis, an entomopathogenic bacterium normally extracted from soil used in biological control, alternative to chemical these insects. Mortality tests must be carried out order select determine a viable toxic strain, but currently there no validated methodology for conducting those tests. In this context, research aimed develop improve selective bioassay assess effect B. thuringiensis Cry toxins on Diceraeus melacanthus (green-belly stink bug) Euschistus heros (neotropical brown nymphs. A consisting tubes artificial diet was proposed. Bioassays with D. E. nymphs were performed incorporating (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, Cry1F, Cry1G, Cry1Ia, Cry2Ab, Cry2A, Cry2Ae, Cry4A, Cry4B, Cry10, Cry11Aa) into liquid diet. feeding system developed carry bug mortality conducted. Among tested, we can highlight 2 causing 80-85% nymphal melacanthus, 4 90-100% after 7 days incubation. Both susceptible different toxins, emphasis Cry2Ab Cry4B Cry1Ia heros.